Composite

Part:BBa_K4652011:Design

Designed by: YUAN-AN CHEN   Group: iGEM23_Mingdao   (2023-08-15)


T7-RBS-SpyTag (D7A)-PCLase1-SpyCatcher-Tr


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

The DNA sequence for PCLase1 is from a Lipase in Pseudomonas hydrolytica with deletions of start codon (ATG) and stop codon (TAA). SpyTag and SpyCatcher are from CnaB domains of fibronectin-binding (FbaB) protein in Streptococcus pyogenes. SpyTag was mutated at Asp7 with Ala (D7A).

References